Csn5 antibody
WebNov 14, 2014 · The lysates were immunoprecipitated with anti-Flag antibody and immunoblotted with indicated antibodies. (f) CSN6 antagonized CSN5 in regulating Cullin-1 neddylation. U2OS cells were infected with ... WebOct 9, 2024 · The changes in abundance of CSN4 and CSN5 proteins in CSN4- or CSN5-silenced tomato lines at 24 h after infection with Meloidogyne incognita. (A) Immunoblot analysis of CSN4 and CSN5 protein levels in roots of tobacco rattle virus (TRV)-CSN4 and TRV-CSN5 and control plants using anti-CSN4 and anti-CSN5 antibodies. Equal protein …
Csn5 antibody
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WebJan 15, 2008 · CSN5 has been implicated as a candidate oncogene in human breast cancers by genetic linkage with activation of the poor-prognosis, wound response gene expression signature. CSN5 is a subunit of the eight-protein COP9 signalosome, a signaling complex with multiple biochemical activities; the mechanism of CSN5 action in cancer … WebCSN5 Antibody (IHC-00362) in IHC. Detection of human CSN5 by immunohistochemistry. Sample: FFPE section of human islet cell tumor. Antibody: Affinity purified rabbit anti-CSN5 (Cat. No. IHC-00362) used at a dilution of 1:250. Detection: DAB.
WebNov 6, 2024 · PD-L1 is an essential immune checkpoint protein that binds to PD-1 on T cells, which plays a critical role in killing cancer cells, while cancer cell exhibits immune escape by the expression of PD ... WebFeb 2, 2024 · JAB1 Antibody (B-7) is a high quality monoclonal JAB1 antibody (also designated CSN5 antibody, COP9 Signalosome Subunit 5 antibody, JAB1 antibody, SGN5 antibody, Jun Activation Domain-Binding Protein 1 antibody, COP9 Signalosome Complex Subunit 5 antibody, or Signalosome Subunit 5 antibody) suitable for the …
WebPolyclonal Antibody for studying COPS5. Cited in 9 publications. Validated for WB, IP, IF. Highly specific and rigorously validated in-house, COPS5 … WebJun 17, 2024 · A. co-IP between endogenous CSN5 and ZEB1 in ACHN cells. ZEB1 was detected in the immunoprecipitate when the anti-CSN5 antibody was used as bait. B, colocalization studies were performed on ACHN and A498 cells using CSN5 antibody (1:200, green) and anti–ZEB1 antibody (1:200, red), followed by DAPI nuclear …
WebThe lysates were also immunoprecipitated with CSN6 antibody or CSN5 antibody and were subjected to immunoblotting with anti-Cullin-1. (d) MPN domain of CSN6 had an important role in competing with CSN5 for Cullin-1 binding and affected Cullin-1 neddylation. 293T cells were cotransfected with indicated plasmids.
WebThe precipitates were immunoblotted with the anti-COX-2 and the anti-CSN5 antibodies. As seen in Fig. 3c, COX-2 co-precipitated with the CSN at a molar ratio of 1:1 demonstrating a direct physical ... pop songs with no bad wordsWebMar 17, 2003 · All western blots shown in Figure 1 were performed with the anti-PKD C-20 antibody from Santa Cruz. The anti-5/6-kinase antibody was a gift from M.Wilson. The anti-CSN5 antibody was obtained from B.Christy and the anti-Cul1 from Calbiochem. All blots were developed by ECL or ECL-Plus technique (Amersham). Cell culture shark and mermaid birthday partyWebDetection of human CSN5 by western blot. Samples: Whole cell lysate (50 µg - E; 25 µg - T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T).Antibody: Affinity purified rabbit anti-CSN5 antibody A300-014A used at … pop songs with mandolinWebDec 4, 2024 · Cells were incubated overnight with anti-CSN5 and anti-PD-L1 primary antibody. After washing, cells were stained with a matching Alexa Fluor 488- or 594-conjugated secondary antibody (Life ... shark and marineWebRabbit anti-CSN5 Antibody. Sign In Create an Account (0) Support Support. Solutions Back. for RESEARCHERS ... Custom Polyclonal Antibodies Custom Antibody … pop songs with maracasWebCLN5 disease is caused by mutations in the CLN5 gene, which provides instructions for making a protein whose function is not well understood. After the CLN5 protein is … shark and mermaidWebNov 30, 2024 · The CSN5 gene was overexpressed or silenced with lentiviral vectors in HCC cells. Cell proliferation was measured using CCK8 assay. ... The membrane was blocked by 5% nonfat milk in TBST, and then incubated with the primary antibody. The antibodies used were as follows: anti-CSN5 antibody (1:500, Abcam, USA), SCARA5 … shark and mermaid party snacks