SpletIn PCR-2, the 18S rRNA gene of H. meleagridis was amplified using primers described previously (Table 1).1 Cycling parameters for the amplification were as follows: 1 cycle of 95°C for 1 min; 35 cycles of 95°C for 15 s, 53°C for 15 s, and 72°C for 1 min; followed by final elongation step at 72°C for 10 min. In PCR-3, novel detection primers to Splet14. maj 2024 · A CCK-8 assay was used to detect chicken preadipocyte proliferation. When the confluence of the passaged chicken primary preadipocytes reached 30, 50, 70, 90, …

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SpletFrequently, histomonosis in chickens appears together with an Escherichia coli infection characterized by salpingitis and peritonitis ( Figure 2 ). Therefore, it is advisable to perform a complete post mortem investigation with special … Splet01. okt. 2024 · The PCR was performed using the following reaction conditions: 95 °C for 3 min, 35 cycles of 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 1 min, and 72 °C for 5 min. Positive and negative controls were included in all runs. 2.4. Gel electrophoresis and sequencing of PCR products teadus ja haridusministeerium

Histomoniasis - European Food Safety Authority

Splet01. jan. 2007 · In the present investigation PCR assays were developed for the rapid detection and differentiation of two poultry flagellates: Histomonas meleagridis and … SpletPathologic Characterization of Coinfection with Histomonas meleagridis, Marek's Disease Virus, and Subtype J Avian Leukosis Virus in Chickens Pathologic Characterization of Coinfection with Histomonas meleagridis, Marek's Disease Virus, and Subtype J Avian Leukosis Virus in Chickens SpletThe PCR products were analyzed on a 1.5% agarose gel as described above. To confirm the PCR-amplified targets, gel bands were sliced and DNA was purified by using Gel Extraction Kit (TaKaRa, Daliang, China). Pure DNA was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and transformed into Escherichia coli cells. The selected clones teadus pharma